![]() ![]() Proc Natl Acad Sci U S A 113(8):E968–E977Īgeberg M, Lindmark A (2003) Characterisation of the biosynthesis and processing of the neutrophil granule membrane protein CD63 in myeloid cells. Kowal J, Arras G, Colombo M, Jouve M, Morath JP, Primdal-Bengtson B, Dingli F, Loew D, Tkach M, Thery C (2016) Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes. Curr Protoc Cell Biol Chapter 3:Unit 3.22 Thery C, Amigorena S, Raposo G, Clayton A (2006) Isolation and characterization of exosomes from cell culture supernatants and biological fluids. Witwer KW, Buzas EI, Bemis LT, Bora A, Lasser C, Lotvall J, Nolte-‘t Hoen EN, Piper MG, Sivaraman S, Skog J, Thery C, Wauben MH, Hochberg F (2013) Standardization of sample collection, isolation and analysis methods in extracellular vesicle research. Each protein standard or ladder is supplied in a ready-to-use format, eliminating the need to reduce, pre-mix, or add loading. Gould SJ, Raposo G (2013) As we wait: coping with an imperfect nomenclature for extracellular vesicles. Protein ladders and standards for SDS-PAGE, western blots, and isoelectric focusing (IEF) Choose from a variety of protein ladders (molecular weight markers) for protein electrophoresis and western blotting applications. Recommended volume per well for 1.0 mm mini gelġ0 μL for electrophoresis, 5 μL for blottingĥ μL for electrophoresis, 2.Tkach M, Thery C (2016) Communication by extracellular vesicles: where we are and where we need to go. Store the prepared solution at either 4C or room temperature, but ensure you protect it from light. The SeeBlue Plus2 Pre-Stained Standard allows you to easily visualize protein molecular weight ranges during electrophoresis and quickly evaluate Western transfer efficiency. The final concentration will be 5 v/v glacial acetic acid and 0.1 w/v Ponceau S. Hence, you do not need to add reducing agent. ~160, 120, 80, 60, 50, 40, 30, 20, 15, 10 kDaĮach protein standard contains a 6X His-tag enabling the detection with the InVision His-tag In-gel StainĬolorimetric or methods that detect phosphorylated proteins such as Pro-Q Diamond phosphoprotein gel stainsĬolorimetric or methods that detect glycosylated proteins such as Pro-Q Glycoprotein stain kits To make a 100 mL solution from the powder, weigh 100 mg of Ponceau S powder and make it up to 95 mL with distilled water before adding 5 mL of glacial acetic acid. Except for our NativeMark Unstained Protein Standard (designed for native electrophoresis), all of the other unstained and prestained standards we offer (Novex Sharp, SeeBlue, SeeBlue Plus2, BenchMark, HiMark) have been pre-reduced (by a proprietary method). Peppermint Stick Phosphoprotein Molecular Weight StandardsĬand圜ane Glycoprotein Molecular Weight Standards Tagged- proteins in the 10 -250 kDa range contain a Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II sequenceĭirect visualization can be achieved through standard staining protocols (e.g., Coomassie, etc.) Tagged-proteins contain an integral Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II sequence SKAP1 src kinase associated phosphoprotein 1. PageRuler Unstained Broad Range Protein LadderĪccurate estimation across a broader range blot showing a p53 and p21 dose response in a panel of MCL cell lines. These protein standards and ladders are consistent from lot to lot and are strictly. Each protein standard or ladder is supplied in a ready-to-use format, eliminating the need to reduce, pre-mix, or add loading dyes. Recommended Volume per well for 1.0 mm gel (μL)Ĭolorimetric, NIR fluorescence (700nm channel, blue bands), RGB fluorescence (550 nm channel, orange bands) Choose from a variety of protein ladders (molecular weight markers) for protein electrophoresis and western blotting applications. Visible monitoring of gel separation and transfer efficiency Compare and view all other protein standards and ladders Applications Western blotting: detection of the nine unstained bands via the detection method used for the target protein. Method: Western blot analysis of EGFR was performed by loading serially diluted A431 cell lysate onto a NuPAGE 38 Tris-acetate gel (Cat. Spectra Multicolor Broad Range Protein Ladderīest multicolor prestained ladder for routine applicationsĤ colors for improved visualization during separation and transferĪnalysis of high molecular weight proteins The protein standard is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add sample buffer prior to use. Western blotting analysis of EGFR expression in A431 lysates transferred from an Novex 420 Tris-glycine gel and a NuPAGE 38 Tris-acetate gel using the iBlot 2 Gel Transfer Device. ![]()
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